Targeted Chromatin Profiling Reveals Novel Enhancers in Ig H and Ig L Chain Loci

Targeted chromatin profiling reveals novel enhancers in Ig H and Ig L chain Loci.

The assembly and expression of mouse Ag receptor genes are controlled by a collection of cis-acting regulatory elements, including transcriptional promoters and enhancers. Although many powerful enhancers have been identified for Ig (Ig) and TCR (Tcr) loci, it remained unclear whether additional regulatory elements remain undiscovered. In this study, we use chromatin profiling of pro-B cells to...

The Multiple Shark Ig H Chain Genes

Immature surface Ig+ B cells can continue to rearrange kappa and lambda L chain gene loci

Pro and pre B cells possess the long-term capacity to proliferate in vitro on stromal cells and interleukin 7 (IL-7) and can differentiate to surface immunoglobulin (sIg+) cells upon removal of IL-7 from the cultures. A key event in this differentiation is the extensive cell loss due to apoptosis. Because the proto-oncogene bcl-2 can promote cell survival, we established pre-B cell lines from E...

Autonomously The Multiple Shark Ig H Chain Genes

Hypermutate Autonomously The Multiple Shark Ig H Chain Genes